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Why Mitalipov’s ‘magic’ room for human embryo CRISPR may give us pause

October 3, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 7 Comments

The debate over whether the main conclusions of the Nature paper on human embryo CRISPR led by Shoukhrat Mitalipov’s lab at Oregon Health Sciences University (OHSU) are correct remains unresolved. Note that Nature just added an editorial alert just above the references section to their paper: “Updated online 02 October 2017. Editorial Note: Readers are alerted that some …

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Mulling over that Mitalipov human embryo CRISPR pub, still many questions

September 12, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 6 Comments

What really happened at the DNA level in the experiments in that high-profile CRISPR of human embryos paper from a team led by Shoukhrat Mitalipov at OHSU? Is the team right that they successfully conducted CRISPR of human embryos to correct a mutant gene, as they reported in their Ma, et al. Nature paper? Or is the Egli, …

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Mitalipov briefly responds to Egli preprint, stands by human embryo CRISPR pub findings

August 31, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 16 Comments

There has been a wave of intense discussions both in the public domain such as on Twitter and behind the scenes over the new Egli, et al. preprint that challenges the main conclusions of the Ma, et al. Nature paper from Shoukhrat Mitalipov’s lab. Ma, et al. reported CRISPR gene editing of human embryos, arguing for …

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Big 5 questions anticipating Mitalipov human embryo CRISPR paper

July 31, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D.

The human embryo CRISPR paper with Shoukhrat Mitalipov as senior author is coming soon. It will reportedly be focused on the use of CRISPR to genetically modify viable human embryos for reversal of a disease-associated mutation. While strangely press already broke early on this paper last week, as much as a week before the paper …

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Human embryo CRISPR pub includes plagiarism: the victim’s unique account

May 18, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 5 Comments

Cut, modify, paste… It’s kind of a CRISPR mantra for those of us using gene editing in the lab. But it’s supposed to be happening just on DNA, right? Now it appears that someone on a team of human embryo CRISPR researchers possibly got carried away with the cutting-modifying-pasting mindset to take it beyond DNA to also do …

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CRISPR often damages human embryo chromosomes

July 1, 2020 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 3 Comments

Three new studies reported in preprints all show severe DNA damage to human embryos from CRISPR a surprisingly high percentage of the time including in some cases loss of heterozygosity (LOH). Anywhere from about a quarter to half of CRISPR’d embryos exhibited major genome damage. As readers of The Niche know, I never was a …

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Why we shouldn’t view the human embryo as a gizmo even in the CRISPR era

January 31, 2018 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 4 Comments

My first job in science was as a lab technician at UCSD School of Medicine and a big part of that job was growing cells called HUVECs or human umbilical vein endothelial cells. We isolated and grew the HUVECs from umbilical cords that we retrieved from the maternity ward of the UCSD hospital, which first …

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1st Knockout Human Embryos Made with CRISPR: My Take on the Pub

September 20, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 4 Comments

Fogarty-Fig-3d-human-embryo-CRISPR-e1505774206656

Scientist make knockout human embryos with CRISPR? Today we see a new Nature paper (Fogarty, et al.) on CRISPR “gene editing” of human embryos, this time from the UK from Kathy Niakan’s group. Niakan got UK permission about 18 months ago to CRISPR healthy human embryos so they’ve been hard at work since. Because Fredrik …

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Doubts raised on key points of Nature paper on CRISPR gene editing of human embryos

August 30, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 11 Comments

Is it possible that CRISPR gene editing actually didn’t happen in many of the human embryos in that big Nature paper that made such news a couple weeks back? Some doubts have emerged that call the main conclusions of the paper into question and argue that more definitive studies are needed to be sure. An …

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Review of Mitalipov paper CRISPR’ing human embryos: transformative work on the edge

August 2, 2017 February 1, 2021 / By Professor Paul Knoepfler, Ph.D. / 3 Comments

In the same way perhaps that some excited relatives or parents-to-be both gush and worry about a baby before it is even born, our field has been transfixed for a week by the Mitalipov paper on CRISPR’ing human embryos even though the paper just now came out. Now that the paper is out, we can …

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