May 29, 2020

The Niche

Knoepfler lab stem cell blog

CRISPR off-target

4 min read

If it seems to you like dramatic cautionary tales about CRISPR accompanied by all sorts of media are coming at us more frequently, it’s not your imagination. In the latest yesterday, it was reported in a new paper led by Allan Bradley that CRISPR-Cas9 results in sometimes large-scale chromosomal lesions at or even away from the target locus. The media have really sunk their teeth into this one, just as in past situations where CRISPR has reportedly encountered anything from bumps in the road to …Read More

3 min read

A new, brief study in mice from the Vinit Mahajan lab argues CRISPR can have very large numbers of off-target sites. Their paper is entitled “Unexpected mutations after CRISPR–Cas9 editing in vivo” and was published in Nature Methods.This work has garnered a lot of attention in the media. Let’s take a journal club review kind of approach to this pub. In this paper there were two major reported findings. First, the number of reported CRISPR-associated mutations in the two mice studied (versus one control) were high, over a …Read More