September 25, 2020

The Niche

Knoepfler lab stem cell blog

Gaetan Burgio

4 min read

If it seems to you like dramatic cautionary tales about CRISPR accompanied by all sorts of media are coming at us more frequently, it’s not your imagination. In the latest yesterday, it was reported in a new paper led by Allan Bradley that CRISPR-Cas9 results in sometimes large-scale chromosomal lesions at or even away from the target locus. The media have really sunk their teeth into this one, just as in past situations where CRISPR has reportedly encountered anything from bumps in the road to …Read More

4 min read

Some in CRISPR-Cas9-land who are focused on potential future clinical applications are kind of rejoicing or at least sighing a breath of relief. This upbeat swing in the atmosphere (from investors especially) was sparked by retraction of that paper, the one initially reporting tons of supposed off-target CRISPR-Cas9 activity in mice, which turned out to be a “nothing burger” according to one investment site. Off-target activity definitely still needs to be on people’s radar screens, but it’s a problem that’s not nearly so widespread as that paper incorrectly …Read More

6 min read

Is it possible that CRISPR gene editing actually didn’t happen in many of the human embryos in that big Nature paper that made such news a couple weeks back? Some doubts have emerged that call the main conclusions of the paper into question and argue that more definitive studies are needed to be sure. An international team of top scientists led by first author Dieter Egli has responded via a preprint on Biorxiv to that Mitalipov team high-profile Nature paper on CRISPR gene editing of …Read More

3 min read

A new, brief study in mice from the Vinit Mahajan lab argues CRISPR can have very large numbers of off-target sites. Their paper is entitled “Unexpected mutations after CRISPR–Cas9 editing in vivo” and was published in Nature Methods.This work has garnered a lot of attention in the media. Let’s take a journal club review kind of approach to this pub. In this paper there were two major reported findings. First, the number of reported CRISPR-associated mutations in the two mice studied (versus one control) were high, over a …Read More