September 29, 2020

The Niche

Knoepfler lab stem cell blog

human embryo

5 min read

When I was a little kid I played this game called “connect the dots” or “dot-to-dot”, where you draw a line from dot-to-dot in numerical order and at some point a picture starts to emerge. I think kids and even some adults still play this today. They even come in extreme versions with hundreds or thousands of dots now (see image of books). I always tried to guess what the picture would be as soon as possible and sometimes even before starting to draw …Read More

4 min read

Remember that Shoukhrat Mitalipov lab paper on the use of CRISPR in human embryos? It’s back in the news. One of the biggest stories of 2017 centered on a Nature paper (Ma, et al., see my quick, initial review shortly after it was published here) from Mitalipov’s lab claiming both efficient repair of a disease-causing mutation in human embryos via CRISPR-Cas9 gene editing and that the correction happened at the MYBPC3 gene via an unexpected mechanism using the normal maternal chromosome as a template (the …Read More

4 min read

My first job in science was as a lab technician at UCSD School of Medicine and a big part of that job was growing cells called HUVECs or human umbilical vein endothelial cells. We isolated and grew the HUVECs from umbilical cords that we retrieved from the maternity ward of the UCSD hospital, which first entailed getting the placenta and attached umbilical cord as our starting material. Some people viewed this material as “gross”, but as a newbie scientist I was kind of in …Read More