August 8, 2020

The Niche

Knoepfler lab stem cell blog

NgAgo versus CRISPR

1 min read

The supposed new gene editing technology NgAgo has had the scissors taken to it with basically no one able to get it to work conclusively and now the professor who led the work, Chunyu Han, has posted a new NgAgo protocol on the Addgene website. Updated NgAgo gene-editing: scientist posts revised protocol, with skeptical response https://t.co/Lt2tc9ClET #CRISPR #China #geneediting — David Cyranoski (@Cyranoski) August 10, 2016 The new NgAgo protocol is really more of an abbreviated methods description with just a few new hints …Read More

2 min read

Does the new gene editing method NgAgo work or not? If not, what happened? The answers to both questions seem to depend on who you ask and what you read. As much as CRISPR has been the revolutionary in the genetic modification technology arena over past methods, could CRISPR itself in the next few years become obsolete having been replaced by other new technologies such as the upstart NgAgo? I doubt it. The odds for NgAgo making a run in this field may have …Read More

3 min read

The gene editing technology CRISPR has been arguably the top story in the biomedical world in the last two years, but going forward there is a CRISPR challenger in upstart gene editing technology NgAgo. For more background on NgAgo and the key first published paper on its genetic modification characteristics see my post here.  In the comments on that post and in discussions I’ve had with other researchers, some key points have crystalized on NgAgo versus CRISPR at this time. As a possible CRISPR challenger, how does NgAgo fare? …Read More

2 min read

2020 Update: early NgAgo reports have been mostly discredited and there is doubt on its function as a gene-editing method. What could be better than CRISPR for gene editing? A new genetic modification technology called NgAgo has some researchers really excited. How does it compare to CRISPR? I’ll admit it that as a scientist who works on genetics and genomics, I am really enjoying the power and simplicity of CRISPR-Cas9 type technology for genome editing. We are working with it extensively in my lab. One of …Read More