July 4, 2020

The Niche

Knoepfler lab stem cell blog

Somatic Cell Nuclear Transfer

4 min read

Advances in therapeutic cloning reported in the past year have been very exciting. Somatic cell nuclear transfer (SCNT) can be used to produce very powerful human embryonic stem cells (ESC). These new cells are called NT-ESCs for short. Neither embryos nor reprogramming factors are needed to produce human NT-ESCs. See here, here and here for discussions of the pioneering papers reporting creation of NT-ESC including the first paper by the lab of Shoukhrat Mitalipov of OHSU, which I called the stem cell event of the year for 2013. Now that human NT-ESC are …Read More

4 min read

Just how good are human embryonic stem (ES) cells made by therapeutic cloning via nuclear transfer? How do they compare to induced pluripotent stem (iPS) cells or traditional ES cells made from IVF embryos? A new paper in Nature directly tackles these key questions, but first a bit of context. Three separate groups have now successfully made ES cells using somatic cell nuclear transfer (SCNT), with the successful technique first reported by the lab of Shoukhrat Mitalipov at OHSU last year. I have reviewed those three therapeutic cloning papers …Read More

3 min read

For the first time ever, scientists have successfully used somatic cell nuclear transfer (SCNT) via the process of therapeutic human cloning to generate normal human embryonic stem cells (hESC). Recall that there are two kinds of human cloning: therapeutic (which is reported in the new paper discussed in this post) and reproductive, which is making an actual new person with an identical genome to an existing person. The latter has never been achieved, but some of us are worried it is coming sooner than …Read More

1 min read

I just closed my blog’s poll on what are the most clinically promising pluripotent stem cells. Why close the poll now less than 12 hours after I opened it instead of leaving the poll open longer? You’ll find out soon enough. I can’t resist teasing you readers sometimes. iPS cells were the big winners in the poll at 48%. Allogeneic human ES cells were 2nd at 28%. In contrast, not a single person voted for SCNT-derived human ES cells. Pluripotent adult stem cells finished …Read More