July 10, 2020

The Niche

Knoepfler lab stem cell blog

Search Results for: NgAgo

1 min read

The supposed new gene editing technology NgAgo has had the scissors taken to it with basically no one able to get it to work conclusively and now the professor who led the work, Chunyu Han, has posted a new NgAgo protocol on the Addgene website. Updated NgAgo gene-editing: scientist posts revised protocol, with skeptical response https://t.co/Lt2tc9ClET #CRISPR #China #geneediting — David Cyranoski (@Cyranoski) August 10, 2016 The new NgAgo protocol is really more of an abbreviated methods description with just a few new hints …Read More

2 min read

When potentially game changing new technologies are reported such as NgAgo gene editing, both scientists and the public get excited, but especially if such new reports stem from a single paper it is wise to take a cautious approach for a while. The key question is whether the new findings will turn out to be reproducible. With the case of NgAgo specifically, the Nature Biotechnology paper reporting potentially very desirable gene editing properties, drew a lot of interest. See archived blog posts on NgAgo here. …Read More

8 min read

By Gaetan Burgio CRISPR/Cas9 genome editing has dramatically changed our way to perform biological experiments. While highly efficient and easy to use, one limitation with CRISPR/Cas9 mediated genome editing technology is the occurrence of off-target effects and the restriction of the PAM recognition sequence. Many modifications from the original system have been proposed to improve its efficiency, specificity and to avoid off-target effects. Recently a new system based on the bacteria Natronobacterium gregory Argonaute (NgAgo) was proposed as a serious alternative to CRISPR/Cas9. NgAgo …Read More